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dna sequencing service  (Macrogen)

 
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    Macrogen dna sequencing service
    Dna Sequencing Service, supplied by Macrogen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+sequencing+service/sequencing/pm42281337-264-7-12
    Average 86 stars, based on 1 article reviews
    dna sequencing service - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    DNA Sequencing:

    Article Title: Semi-Rational Engineering of Formate Dehydrogenase Reveals Loop Dynamics as a Key Determinant of Thermostability.
    Article Snippet: Formate dehydrogenase (FDH) catalyzes the reversible formate oxidation using NAD+ to yield carbon dioxide and NADH.. FDH is widely utilized for cofactor regeneration, enabling the continuous supplying of NADH for chiral chemical biosynthesis.. Herein, we employed semi-rational engineering to improve the thermostability of highly catalytic FDH from Bacillus simplex (BsFDH).

    Article Title: Contrasting genetic diversity and ecological niche modelling of the montane grass mouse Akodon montensis in the south of the Atlantic Forest
    Article Snippet: .. PCR products were purified with an Accuprep purification Kit ( Bioneer ) and sent to the DNA sequencing service of Macrogen Inc. All mitochondrial sequences obtained were submitted to the GenBank database under accession number PP715535 to PP715628 (electronic supplementary material, table S2). ..

    Article Title: Respiration and Substrate Utilization by Marine Fungi: Their Ecological Role in an Upwelling Ecosystem
    Article Snippet: .. The PCR products were sequenced for identification analysis at the Automated DNA Sequencing Service of MACROGEN (Seoul, South Korea; http://dna.macrogen.com/eng ). .. Genera and species were identified by matching sequences with those stored in GenBank based on previous ITS rDNA definitions in fungi using BLAST (National Center for Biotechnology Information (NCBI)).

    Article Title: Synergistic antimicrobial and probiotic activity of lactic acid bacteria isolated from Tarkhineh against Candida albicans.
    Article Snippet: .. The amplified products were then sequenced by Macrogen DNA sequencing service (Korea), aligned using the Clustal W program, and compared to reference bacterial strains in the GenBank databases12,13. ..

    Article Title: Synergistic antimicrobial and probiotic activity of lactic acid bacteria isolated from Tarkhineh against Candida albicans
    Article Snippet: .. The amplified products were then sequenced by Macrogen DNA sequencing service (Korea), aligned using the Clustal W program, and compared to reference bacterial strains in the GenBank databases , . .. To reduce the number of bacterial strains for further study, an initial evaluation was conducted by measuring the optical densities (OD) of the strains under acidic (pH 2.5 at 37 °C) and bile (pH 6.8 at 37 °C with 0.3 w/v % concentration of oxgall: dehydrated fresh bile, Becton and Dickinson) conditions.

    Article Title: Quercetin triggers cell apoptosis-associated ROS-mediated cell death and induces S and G2/M-phase cell cycle arrest in KON oral cancer cells.
    Article Snippet: VISafe green gel stain was used to color the gels, and the gel document (Alliance Q9 Advanced, UVITEC, Cambridge, UK) was employed to determine the densities of the DNA bands. .. The Macrogen DNA Sequencing Service, located in Thailand, handled the primer sequencing. ..

    Article Title: In Vitro Antifungal Activity of Amphotericin B-Encapsulated Silk Fibroin-Chitosan Nanoparticles Against Fusarium solani Isolates from Keratitis Patients
    Article Snippet: .. 18S rRNA gene sequences and their associated transcribed spacers (internal transcribed spacer; ITS) were performed by the DNA Sequencing Service of Macrogen (Seoul, Republic of Korea). ..

    Polymerase Chain Reaction:

    Article Title: Contrasting genetic diversity and ecological niche modelling of the montane grass mouse Akodon montensis in the south of the Atlantic Forest
    Article Snippet: .. PCR products were purified with an Accuprep purification Kit ( Bioneer ) and sent to the DNA sequencing service of Macrogen Inc. All mitochondrial sequences obtained were submitted to the GenBank database under accession number PP715535 to PP715628 (electronic supplementary material, table S2). ..

    Article Title: Respiration and Substrate Utilization by Marine Fungi: Their Ecological Role in an Upwelling Ecosystem
    Article Snippet: .. The PCR products were sequenced for identification analysis at the Automated DNA Sequencing Service of MACROGEN (Seoul, South Korea; http://dna.macrogen.com/eng ). .. Genera and species were identified by matching sequences with those stored in GenBank based on previous ITS rDNA definitions in fungi using BLAST (National Center for Biotechnology Information (NCBI)).

    Purification:

    Article Title: Contrasting genetic diversity and ecological niche modelling of the montane grass mouse Akodon montensis in the south of the Atlantic Forest
    Article Snippet: .. PCR products were purified with an Accuprep purification Kit ( Bioneer ) and sent to the DNA sequencing service of Macrogen Inc. All mitochondrial sequences obtained were submitted to the GenBank database under accession number PP715535 to PP715628 (electronic supplementary material, table S2). ..

    Amplification:

    Article Title: Synergistic antimicrobial and probiotic activity of lactic acid bacteria isolated from Tarkhineh against Candida albicans.
    Article Snippet: .. The amplified products were then sequenced by Macrogen DNA sequencing service (Korea), aligned using the Clustal W program, and compared to reference bacterial strains in the GenBank databases12,13. ..

    Article Title: Synergistic antimicrobial and probiotic activity of lactic acid bacteria isolated from Tarkhineh against Candida albicans
    Article Snippet: .. The amplified products were then sequenced by Macrogen DNA sequencing service (Korea), aligned using the Clustal W program, and compared to reference bacterial strains in the GenBank databases , . .. To reduce the number of bacterial strains for further study, an initial evaluation was conducted by measuring the optical densities (OD) of the strains under acidic (pH 2.5 at 37 °C) and bile (pH 6.8 at 37 °C with 0.3 w/v % concentration of oxgall: dehydrated fresh bile, Becton and Dickinson) conditions.

    other:

    Article Title: Identification of a Snf7-domain-containing protein that exhibits high affinity and synergistic activity for Cry13Aa1 toxin in Bursaphelenchus xylophilus.
    Article Snippet: Pine wilt disease, caused by the pinewood nematode Bursaphelenchus xylophilus (Rhabditida: Aphelenchoididae), results in significant global economic and ecological impacts.. Although the Cry13Aa1 toxin from Bacillus thuringiensis shows nematicidal activity, its mechanism of action against B. xylophilus remains unclear.. This study aimed to identify and characterize the receptors for Cry13Aa1 in B. xylophilus.

    Sequencing:

    Article Title: Quercetin triggers cell apoptosis-associated ROS-mediated cell death and induces S and G2/M-phase cell cycle arrest in KON oral cancer cells.
    Article Snippet: VISafe green gel stain was used to color the gels, and the gel document (Alliance Q9 Advanced, UVITEC, Cambridge, UK) was employed to determine the densities of the DNA bands. .. The Macrogen DNA Sequencing Service, located in Thailand, handled the primer sequencing. ..



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    (A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus <t>DNA</t> ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense <t>sequence</t> (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).
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    (A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus <t>DNA</t> ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense <t>sequence</t> (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).
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    (A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus <t>DNA</t> ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense <t>sequence</t> (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).
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    (A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus <t>DNA</t> ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense <t>sequence</t> (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).
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    (A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus DNA ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense sequence (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).

    Journal: Bio-protocol

    Article Title: Plasmid Curing of Pseudoalteromonas haloplanktis TAC125 Using Homologous Recombination and PTasRNA Gene Silencing

    doi: 10.21769/BioProtoc.5687

    Figure Lengend Snippet: (A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus DNA ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense sequence (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).

    Article Snippet: DNA sequencing service (e.g., Eurofins Genomics, Ebersberg, Germany) 7.

    Techniques: Agarose Gel Electrophoresis, Plasmid Preparation, Negative Control, Cloning, Sequencing, Marker